Facilities

NanoDrop Lite

The NanoDrop Lite performs basic microvolume measurements. The surface tension allows sample to be pipetted directly onto the optical measurement surface. After measurement the sample is wiped off the measurement surface with a lint free lab wipe.

• Measures nucleic acid concentration at 260nm and purity using the 260/280 ratio
• Measures purified protein concentration at 280nm
• Employs the unique NanoDrop microvolume sampling technology
• Delivers the accuracy and reproducibility expected from NanoDrop instruments
• Uses built-in controls and software–no computer required
• Optional accessory printer for cryogenic labels available

Mic Real-Time PCR System

Mic is a very compact, 48-wells, light-weight real-time PCR cycler that can complete runs in under 25 min, ensuring fast and highly accurate results.

The system is very portable and truly plug and play and does not require calibration ever!

It uses a patented magnetic induction technology to heat your samples and fan forced air for cooling.

The Mic tube format uses 0.1 mL strips of four tubes and matching caps, supporting volumes of 5 to 30 µL. A tab on the first tube ensures correct orientation of tubes at all times.

Applications: Relative Quantification, Absolute Quantification, Genotyping, and Presence/Absence.

ABI^™ 7500 Real-Time PCR Instrument-Applied Biosystems

The Applied Biosystems 7500/7500 Fast Real-Time PCR System is a 96-well, five-color platform that uses fluorescence-based polymerase chain reaction (PCR) reagents to provide:

• Quantitative detection of target nucleic acid sequences (targets) using real-time analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs post-PCR)
• The powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™/SYBR® Green I, VIC®/JOE™, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes
• The specialized optical system enables easy and accurate calibration to accommodate new dyes without the addition of new filter sets
• Advanced multi-component algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate setup wizards, multiple data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
• Thermal cycling block (size 0.2ml) is built into the body of the system to reduce environmental exposure and contamination risk

QuantStudio^™ 5 Dx Real-Time PCR System

The Applied Biosystems™ QuantStudio™ 5 Dx Real-Time PCR System an in vitro diagnostic (IVD) quantitative PCR system is designed with the clinic in mind—delivering a modern, interactive diagnostic instrument. . The QuantStudio 5 Dx instrument incorporates multimode software that allows it to be used in development mode for assay development, and in IVD mode for routine in vitro diagnostic testing. The QuantStudio 5 Dx system is well suited for any clinical laboratory.

• Detect differences in target quantity as small as 1.5-fold in singleplex reactions and obtain 10 logarithmic units of linear dynamic range
• Thermal block format: 96-well (0.2-mL block)
• Excitation source: Bright white LED
• Filter or color combinations: 6 decoupled filters, CMOS camera
• Excitation/detection range: 450–680 nm/500–730 nm
• Multiplexing: 5-plex + 1 passive reference, 6-plex with no passive reference
• Compatible dyes: FAM, SYBR Green, VIC, ABY, NED, TAMRA, JUN, ROX, Mustang Purple, Cy5
• Multiplexing and filters: Up to 6 targets; 21 filter combinations

GENEAMP 9700 PCR SYSTEMS- Applied Biosystems

The Applied Biosystems GeneAmp 9700 PCR system is specifically designed for high sample volume amplification of nucleic acids. The heated lid and unique thermal isolation frame ensures oil free operation in the 0.5µl format. These PCR’s are designed for use with 0.2ml reaction tubes/plates for all routine PCR applications. It features an easy to use graphic interface with intuitive programing, and fast, uniform thermal response.

iBright™ CL1000 Imaging System

The iBright™ CL1000 Imaging System provides for imaging of chemiluminescent Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and visible protein gels with high resolution images. The instrument can be run directly from the touchscreen to start and create images, while also providing on-board software for image analysis.

• Clear interface layout combined with a 12.1 inch capacitive touchscreen
  • Capture crisp, clear images with a 9.1MP camera with Smart Exposure technology
  • Imaging modes as follow:

    imaging capability	What kind of signal can be captured?
    Protein gel	Colorimetric staining of gels (e.g., Coomassie and silver stains) and membranes (e.g., Ponceau S and MemCode stains)
    Nucleic acid gel	Ethidium bromide and SYBR dye staining
    Chemiluminescent blot	Chemiluminescence detection using all popular horseradish peroxidase (HRP) and alkaline phosphatase (AP) substrates (e.g., SuperSignal and WesternBreeze substrates)

Qsonica

Qsonica is used for shearing Chromatin and DNA, by inserting a small probe directly into a sample, Energy is transmitted from the probe directly into the sample and the entire volume is processed very quickly.

At BEC we have the Q800R3 Sonicator: A Sonicator specially designed for DNA and Chromatin shearing. Obtain highly reproducible fragment sizes with low deviation. Ideal for fragment sizes 150bp to 3kb. Needed volume is 50 µl - 1 ml

ProFlex™ 96-well PCR System and 3x32-well PCR System

The ProFlex™ PCR System is a thermal cycler, That allows you to:

• Run three experiments at once with 3 independently controlled 32-well modules; 0.2-mL block( 3 different users or same user)
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex™ blocks

VeriFlex™ blocks are controlled by multiple Peltier elements (2 separate Peltier elements per 32-well module) and (6 separate Peltier elements per 96-well module) This gives you the ultimate control of your block for precise, 'better-than-gradient' optimization experiments, and for running multiple reaction conditions at once for precise incubations and other non-PCR workflows.

“This machine is not only a gradient PCR but it is a combination of three different PCR machines in one. In this PCR machine, we can run three different protocols anytime. Also, three of each different grid has gradient platform which facilitates even more optimization in a single experiment.”

QuantStudio™ 6 Flex Real-Time PCR System

The systems can detect differences as small as 1.5-fold in singleplex reactions with 10 logs dynamic range.

Interchangeable 96-well (0.1 and 0.2 mL), 384-well, plus TaqMan Array card blocks (only the 0.1ml Block format is available at BRC)

Multiplexing and filters: Up to 5 targets

• Available dyes: FAM,SYBR Green, VIC/JOE, NED/ TAMRA/Cy3, ROX/ Texas Red
• Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
• Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
• The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
• Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

Qubit Fluorometric Quantitation

The Qubit™ 4 Fluorometer is a benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein, as well as for the measurement of RNA integrity and quality using the highly sensitive and accurate fluorescencebased Qubit™ assays. Additionally,You can also use the Qubit™ 4 Fluorometer to directly measure the fluorescence of samples or to create new assays using the MyQubit software preprogrammed into the instrument.

2100 Bioanalyzer Instrument

High-Resolution Automated Electrophoresis of DNA, RNA, and Protein Samples

The system integrates an instrument, data processing software, reagents, and a microfluidic chip specific for DNA, RNA, or protein analysis. It is suitable for next-generation sequencing (NGS), gene expression, biopharmaceutical, and genome editing workflows, delivering highly precise analytical evaluation of various sample types. Advantages of microfluidics-based automated electrophoresis over traditional gel electrophoresis include dramatically reduced sample (1 µL for nucleic acids, 4 µL for proteins) and reagent consumption, significantly faster analysis time, and less hands-on activities during sample preparation and data analysis.

Sizing, quantification, and sample quality control of DNA, RNA, and proteins on a single platform.

Biometra Tadvanced

The TAdvanced Twin allows two different users to run two different PCR protocols at the same time.

The Biometra Twin thermal cycler includes two independent blocks in one instrument. Both multiuser environments as well as users with lower sample numbers but different samples will enjoy this model. The twp-block design and the specific Temperature Optimization Step function support the fast optimization of ideal annealing temperatures.

Its features include:

• The combination of ultra-fast heating and cooling rates and excellent temperature homogeneity for constant conditions across all sample positions, the wide range of exchangeable block modules and the professional user management system.
• Designed for error-free use in versatile laboratory environments: universal block design and heating lid with High-Performance Smart Lid (HPSL) technology for unrestricted use of different plastic containers and plates
• Easily optimize your PCR protocols using the Linear Gradient Function (LGT)

Blue light transilluminators - Biometra (BL STAR 16)

Blue light transilluminators are a quite interesting alternative to UV transilluminators as there is no risk of sample damage during illumination. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelengths around 470 nm. Examples of compatible stains are: SYBR® Green, GelGreen™, SYBR® Safe, SYBR® Gold or SYPRO® Ruby. The amber lid of the instrument allows direct viewing of the fluorescent samples. There is no need for separate glasses. For gels up to size of 16 cm x 20 cm.

CO² Incubator (ECIS Z0) SL Shellab

This incubator is connected to ECIS™ Z-Theta, which is an instrument with a distinct method of accurately measuring cellular behaviors including proliferation, migration, barrier function, attachment. The ECIS Z-Theta capable of a multitude of assays, all done in real time, giving it the edge in impedance-based assays.

Forma Direct Heat CO² Incubator model 361

ThermoScientific Forma Direct Heat CO2 Incubator combine high capacity and dependable performance in an easy to use design, for researchers who prefer the convenience of direct heat temperature control technology.

Heraeus CO² Incubator with hypoxia chamber (D-63450)- Function line

This incubator can facilitate experiment for cells in hypoxic conditions.

Heracell™ 240i CO² Incubator

Clean, reliable and easy to use, with fast recovery characteristics optimizing cell growth and a convenient touchscreen user interface. It has a dual temperature probes with over temperature protection. Pan-less system provides recovery rates up to 5X faster than traditional water pans. Ability to connect an N2 cylinder as well.

It features a ContraCon 90°C moist heat disinfection cycle

• On-demand cycle simplifies cleaning and eliminates the need to separately autoclave components
• Operation is push-button simple and does not require removal of sensors or other components

Invitrogen countess^™ II FL

The Countess™ II FL Automated Cell Counter is a fully automated, 3-channel cell counter and assay platform that uses EVOS™ light cube technology, state-of-the-art optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samples in suspension

,

Tali® Image Cytometer

The Tali™ Image-Based cytometer is perfect for:

• Rapid analysis of GFP/RFP vector transfection efficiency
• Identification of GFP and/or RFP positive cells
• Simple two color apoptosis assays
• Accurate cell viability population analysis
• Quick verification of samples prior to sorting or high-end flow cytometry analysis

Forma Series II Water-Jacketed CO₂ Incubators

Precisely control the CO₂ and temperature with Thermo Scientific™ Forma™ Series II 3110 Water-Jacketed CO₂ Incubators, which combine precise control with a TC sensor. Featuring superior parameter recovery characteristics with innovative, continuous contamination control technology.

Liquid Nitrogen Locator

ThermoScientific liquid nitrogen locator provides outstanding temperature uniformity: samples are stored below -180 degree C, even when less than 2" of liquid nitrogen remains in the vessel. It is with a monitor which provides continuous LED readout of liquid nitrogen level.

Biological Safety Cabinet

Purifier Logic+ Class II, Type A2 Biosafety Cabinets provide personnel, product and environmental protection from hazardous particulates such as agents that require Biosafety Level 1, 2 or 3 containment. Other appropriate applications include work with antineoplastic drugs, genetic material, carcinogens, allergens and additional substances that generate hazardous airborne particulates.

BioTek Cytation 5 Cell Imaging Multimode Reader

Cytation 5 combines automated microscopy and conventional microplate detection platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows.

The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.

Features:

• Information on cell morphology, localization of signal, cell count and more is obtained with Cytation 5’s imaging mode.
• Plate reading: absorbance, fluorescence; luminescence; advanced read modes
• Imaging: fluorescence; phase contrast; HC brightfield; brightfield; color brightfield
• Fully automated microscopy tasks, from slide scanning to time-lapse live cell assays, and flexible hardware increase productivity and reduce manual labor
• Combination of monochromator and filter optics delivers flexibility and performance to microplate assays, and choice of optics allows you to read microplates in a wide range of applications

Available Filters at BRC on this instrument:

• Absorbance filters: Monochromator
• Fluorescence filters available for 3 dyes as follow:

  Description	Excitation	Emission
  DAPI	377/50	447/60
  GFP	469/35	525/39
  Texas Red	586/15	647/57

EVOS® XL Core Imaging System

The EVOS® XL Core Imaging System has two types of controls: manual and onscreen. Manual controls include the illumination wheel, focus knobs, Freeze and Save buttons, objective turret, and the optional mechanical stage X-Y axis knobs. Onscreen controls appear at the bottom of the screen when the mouse rolls over the controls area. Using a mouse is optional; you can operate the EVOS® XL Core Imaging System using only manual controls.

Useful Information

• Location: D128
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Chemiluminescence Imaging GeneGnome

The GeneGnome is a dedicated high performance bio imaging system for chemiluminescence applications. GeneGnome's has the ability to image and produce an analysis of the most complex chemiluminescent filters.

Useful Information

• Location: D127
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

ZEISS SteREO Discovery.V12 Microscope

The motorized components of your SteREO Discovery.V12 are fully integrated into your microscope software ZEN and AxioVision: measure and document your samples reproducibly. Observe your Drosophila or zebrafish embryos, stents or circuit boards with an enhanced three-dimensional impression over the whole 12:1 zoom range. Capture images that are crisp and rich in contrast, have excellent color fidelity and simply contain more information. SteREO Discovery.V12 enhances resolution and contrast in biology and quality assurance. The external touch panel SYCOP controls all essential functions of SteREO Discovery.V12: magnification, focus, contrast, brightness and more.

Useful Information

• Location: D130
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

ZEISS Stemi 508

Equipped with apochromatic optics you acquire images of outstanding image contrast and color accuracy. Its mechanics is designed for heavy workloads. With the large up to 36 mm object field you always keep the overview of your sample. The 8:1 zoom then allows to bring details up to 50× magnification. Add interchangeable optics and observe an area of up to 122 mm. Stemi 508 offers better ergonomics than any other Greenough-type stereo microscope: The low viewing angle of 35° lets you keep a relaxed posture even after hours of work.

Useful Information

• Location: D117
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

IX73 Inverted Microscope

The IX73 inverted microscope system sets new standards in advanced live cell imaging with its compact frame, outstanding optical performance and exceptional flexibility. Manual encoded or semi-motorized options enable a variety of component combinations. The IX73 is available as a one-deck system with a low, ergonomic stage or as a two-deck system with additional expansion capabilities. Both provide the ability to perform a multitude of imaging applications, from fast advanced fluorescence imaging and other demanding techniques to routine testing and documentation.

Useful Information

• Location: D130
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Aquaneering System

The Aquaneering system features a tubular stainless steel welded rack. The open frame design allows maximum visibility without sacrificing structural integrity. The onboard water delivery and drainage system are easily removable for cleaning and maintenance. Individual valves, allowing a variety of flow rates to individual tanks, precisely meter water flow. Drain troughs and piping are constructed from clear PVC for convenient monitoring of waste water flows.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

ZebraBox Revolution

The Zebrabox is the first ever high-throughput monitoring enclosure to analyze zebrafish behaviors. The 4th generation of ZebraBox is a complete system, designed for the high-throughput analysis of zebrafish larvae in multi-well plates with a total control over the experiment environment. ZebraBox is a component of ZebraLab and allows the automated observation and tracking of larval zebrafish, and zebrafish embryos. ZebraBox is capable of analyzing zebrafish larvae in multi-well plates, up to 96 individuals simultaneously.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

ZebraCube

Controlling the environment during a behavioral experiment is the key to success. ViewPoint has developed a new cubicle enclosure to control light, sound and vibration. ZebraCube is an individual apparatus that makes it easy to set your experiments up without requiring a full room.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Picoliter Microinjector (PLI-100A)

The PLI-100A Pico-Injector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

NARISHIGE PC-10 Puller

The method used by this puller is to pull the glass capillary vertically, using the gravitational force of its own weight. For more versatility, two modes are provided: a single pull, which pulls at one stretch, and a double pull in which the setting is changed in mid-process. The PC-10 can therefore produce long, thin micropipettes for injection purposes, and firm microelectrodes for patch clamping. The change of setting in the double pull mode can be preset and then performed automatically, which represents a drastic saving of labor over conventional methods. Digital values, with the maximum output of the electromagnet and heater represented as 100, are shown on the display and the heater output is continually increased, making accurate mass production easier. This puller has several simple features to enhance ease of use, such as the employment of two weights (light and heavy) for tension adjustment.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Shake 'N Bake Digital Incubator

The Shake ?N Bake Incubator shelves provide a gentle up and down motion which is ideal for hybridization and wash procedures as well as conventional incubations. The unit features digital temperature setting and display plus a PID temperature controller for rapid heat-up and excellent temperature stability. Timer not included.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

ZEISS Stemi 2000-C Stereo Microscope

The Zeiss Stemi 2000-C stereo microscope offers a wide variety of standard features and options that will meet the most rigorous application demands. The sharp, distortion-free 3D images produced by the Greenough optical system, along with a 7.7X to 1X click stop or continuous zoom range, sets the Stemi 2000-C Zeiss Microscope apart from other stereo microscopes. The included 10X eyepieces provide a 6.5X to 50X magnification range yielding a field of view from 35.4mm down to 4.6mm. By adding supplemental lenses and 25X eyepieces, magnifications of 1.95X to 250X can be achieved for this Zeiss Microscope.

Useful Information

• Contact
  • Lab Technologist
    Tel : (+974) 4403-5653
    Email: enas.alabsi@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Doppler Signal Digitizer

The Indus Doppler Flow Velocity System is a high frequency, real-time pulsed Doppler measurement device with integrated data analysis software designed for measuring cardiovascular function in small animals. The high sampling rates grant excellent temporal resolution, making this the ideal system for studying fast heart rates and rapid blood accelerations present in small animals.

Useful Information

• Location: D117
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

1588 Hova-Bator Incubator

The Genesis Hova-Bator is pre-set for bird eggs. Simply plug it in, then add water and eggs. Nothing to set up or adjust. This circulated air Hova-Bator comes with a thermometer, plastic bottom liner, and is pre-set to 99. F (38 C). Like all incubators, the 1588 requires a stable room temperature and should not be used in areas like sheds or barns.

Useful Information

• Location: D117
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Egg Incubator (Digital Thermostat Equipped)

Useful Information

• Location: D117
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

BSL3 Lab

BSL3 is a self-sustained facility consist of two isolation labs. It is fully equipped for viral- and bacterial research on risk group 3 pathogens. It is applicable to clinical, diagnostic, teaching, research where work is performed with indigenous or exotic agents that may cause serious or potentially lethal disease through the inhalation route of exposure. The BSL-3 laboratory has special engineering and design features. It has enclosures for aerosol generating equipment, single-pass air, double door entry, HEPA filtration of the exhaust, effluent decontamination, personnel showers, and pass-thru autoclave.

Useful Information

• Location: On the east side of H10 Building
• PI: BRC
• Contact
  • Dr. Hashim Alhussain
    Tel : (+974) 4403-6698
    Email: h.alhussain@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Multiskan™ FC Microplate (ELISA) Reader

The Multiskan FC has a 340–850 nm wavelength range, enabling a wide variety of applications from enzyme kinetic studies to Lowry assays. The instrument provides fast and accurate measurements enabling complete 96-well plate reading in less than seven seconds. The instrument is also equipped with linear shaking as standard.

Features:

• Proven performance and reliable day-to-day results through patented optical design and in-built self-diagnostics
• A broad wavelength range of 340–850 nm; equipped with an eight-position filter wheel with three standard filters, 405 nm, 450 nm and 620 nm, preinstalled
• A comprehensive range of easy-to install additional filters available for order
• Fast and accurate measurement of both 96- and 384-well plates for various throughput requirements
• Ease of use through the large color screen and a variety of language versions
• Visual and logical SkanIt software for comprehensive instrument control and data handling

Recommended for:

Immunoassays (ELISA), Protein assays, Endotoxins, Cytotoxicity and proliferation assays, Enzyme assays, Growth curves

DYNEX DS2 system (2-plate ELISA Processing system)

Is a dynamic, precise and cost-effective processing systems.

• Fully automated open system
• Automated sample distribution, incubation, reagent addition, washing and detection steps of assays. Also can customize assay parameters, assay steps, OD read settings, result calculations, quality control checks and report formats.
• Reagent Pipetting Volume: 20 μL – 1,000 μL
• Sample Pipetting Volume: 10 μL – 250 μL
• Photometric Range: 0.100 – 3.0 OD
• Spectral Range: 405 nm – 690 nm
• Washer Programmable Volumes: 50 µL – 1,000 µL

LUMINEX® 200™- Multiplexing up to 100 analytes in a single well of a microtiter plate

The Luminex 200 Instrument System sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry-based platform. The Luminex 200 system is compatible with ProcartaPlex multiplex immunoassays and QuantiGene Plex multiplex gene expression assays.

the Luminex MAGPIX system can do:

• Multiplexing up to 80 analytes per sample using magnetic microspheres
• Read time: 96-well plate in ≤ 45 min (up to 12,800 tests/hour)

Infinite®; 200 PRO Microplate Reader

The Infinite 200 PRO is a user-friendly and affordable multimode reade. The reader at BRC is a filter-based technology ( F Plex multimode ).The Infinite 200 PRO offers excellent sensitivity, multiplexing capabilities and high format flexibility, including 6- to 384-well microplates, PCR plates, and Tecan's patented NanoQuant Plate™ for low sample volumes.

It can perform Absorbance, fluorescence top and bottom and luminescence reading

The available filters are as follow:

Absorbance filters are:

Slide (1)

• Position 1: 260 nm (5 nm bandwidth)
• Position 2: 280 nm (3 nm bandwidth)
• Position 3: 340 nm (10 nm bandwidth)
• Position 4: 540 (10 nm bandwidth)

Slide (2)

• Position 2: 520 (20 nm bandwidth)
• Position 3: 450 (10 nm bandwidth)
• Position 4: 620 (10 nm bandwidth)

Fluorescence filters are:

Slide (2)

• Position 1:
  • Excitation: 560 (20 nm bandwidth)
  • Emission: 590 (20 nm bandwidth)

Epoch Microplate Spectrophotometer

Epoch™ has a monochromator-base UV-Vis wavelength selection, offering expanded options for the life science laboratory without using interference filters, building in inherent cost savings. With its 200 nm to 999 nm wavelength range and 6- to 384- microplate reading capability, controlled with the Gen5 Data Analysis software interface, Epoch fills a void in today's laboratory through smart design and superior features. This robust, low maintenance microplate spectrophotometer ensures even greater value over time. BioStack and 3rd party automation capability is available with the optional Epoch R model.

Useful Information

• Location: D127
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

Sequencing Unit

The sequencing unit at the Biomedical Research Center has successfully established and deployed three high-throughput sequencing platforms: Illumina-MiSeq, MGI-G50, and ONT-GridION. These sequencing platforms provide massively parallel sequencing experiments on various applications starting from targeted sequencing and metagenomics to whole-genome sequencing. The sequencing unit's mission is to conduct state-of-the-art research in genomics, with a focus on the genomics of local habitats and infectious pathogens. The team specializes in the design and development of protocols that offer rapid and accurate identification and characterization of any pathogen of interest. In complementation with other facilities in the center, we were successfully able to isolate, sequence, and characterize viruses from different origins to serve both research and diagnostic purposes. As we are keen to improve infectious disease research in Qatar, the sequencing unit has extended its services to benefit other researchers at Qatar University, Hamad Medical Corporation, and the Ministry of Municipality and Environment. During the COVID-19 pandemic, the sequencing team has led the national efforts to sequence SARS-CoV-2 and has so far sequenced more than 8000 samples. The team has offered outstanding efforts to strengthen the national efforts to control the introduction and spread of SARS-CoV-2 variants by sharing the sequencing data with local authorities and public databases. The team is actively involved in the development of new techniques for whole bacterial genome sequencing, virus genotyping, and metagenomics. in addition to many other massively parallel genetic assays. We also support other researchers through offering consultations on experiment design including sample preparation, library construction, quality check, and analysis of generated sequencing data.

Useful Information

• Location: D138
• PI: BRC
• Contact
  • Sr. Lab Technologist
    Tel : (+974) 4403-7183
    Email: malmeer@qu.edu.qa
  • BRC Administration
    Tel: (+974) 4403-6818
    Email: BRC@qu.edu.qa

The MiSeq System

The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology responsible for generating more than 90% of the world's sequencing data. the MiSeq System is the ideal platform for rapid and cost-effective genetic analysis.

OXFORD Nanoporetechology (MinION & GridION)

The new generation of sensing technology that uses nanopores - nano-scale holes - embedded in high-tech electronics, to perform precise molecular analyses. The only sequencing technology to combine scalability from portable to ultra-high throughput formats with real-time data delivery and the ability to elucidate accurate, rich biological data through the analysis of short to ultra-long fragments of native DNA or RNA. The sensing platform has the potential to be adapted for the analysis of other types of molecules, for example proteins.

MGISP-960- High-throughput Automated Sample Preparation Platform

The MGISP-960 accomplishes plate to plate transfers for high speed microplate processing applications like library prep, ELISA assays, screening and many more. Equipped for 96 well plate.

MGISP-960 has validated methodology and a library kits including:

• WGS
• Exome/Target Sequencing
• RNA / Transcriptome Sequencing
• NIPT
• PGS / PGD
• PMseq
• Tumor mutation gene detection
• Amplicon Sequencing

Biomic V3

BIOMIC V3 is an open system utilizing digital imaging to automate the reading and CLSI/EUCAST interpretation of clinical microbiology tests and QC from various manufacturers. Systems are customized with optional modules including Broth Microdilution, Disk Diffusion, MIC Strip, Organism ID, Colony Count, Agar Dilution and Urine Screen. BIOMIC V3 provides a digital record of test results and high resolution images.

Autoclave Autester ST dry PV III (J. P.Selecta)

The autoclave Complies with current regulations for pressure vessels and safety standards. It has also a Control panel with temperature and pressure indicator, current program parameters, graphical representation of the process in real time. It can be fully configurable 9 programs.

Omega plate reader- Filter-based multi-mode microplate reader

The FLUOstar Omega is a versatile multi-mode microplate reader with sidderent detection modes: fluorescence intensity (including FRET and TRF),and UV/vis absorbance. It utilizes an ultra-fast UV/Vis CCD-spectrometer for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for all life science applications.

Features:

• UV/Vis Absorbance Spectra– complete spectra or individual wavelengths from 220-1000nm. complete spectra or individual wavelengths from 220-1000nm; Unlike a monochromator, the spectrometer allows you to capture the entire UV/Vis spectrum of a sample in approximately one second per well – no scanning needed.
• Fluorescence Intensity – including FRET and TRF
• For Fluorescence based assays: up to 8 filters for the excitation and up to 8 filters for the fluorescence emission can be installed.
• Upgradable for Luminescence reading
• Upgradeable to Fluostar Polarstar Omega

Available filters at BRC omega plate reader are :

Octet® RED96e System

From screening biologics candidates to uncovering new insights into biophysics and cellular signaling, Octet RED96e system uses a Bio-Layer Interferometry (BLI) to measure binding interactions of small molecules, proteins, antibodies, and even cells, determine specificity, calculate titer, characterize affinity, and more. Assays can be performed over a wider temperature range of 15-40°C on the Octet RED96e system, allowing for kinetics measurement of unstable proteins at lower temperatures, or biologically relevant molecules at physiological temperatures.

Optima™ XE Ultracentrifuge

The Large touch-screen display displays more data. Easy to read and navigate. Viewable from across the lab. Energy efficient, Regenerative braking returns energy to local circuit reducing utility costs. Highly efficient thermoelectric cooling results in lower power consumption. Uses the same amount of power as a 60-watt light bulb while idling.

Milli-Q® Ultrapure Water Solutions | Type 1

Milli-Q® systems offer a unique combination of optimized water purification and monitoring technologies. The selection of the proper ultrapure water system for your laboratory will depend on several parameters such as: feed water available, daily volume needs, monitoring requirements, certification levels expected and any other specific requirements you may have. Application Specialists are available to discuss and help you design the laboratory water system best adapted to your needs.

Stomacher 400 Circulator

Stomacher 400 Circulator is blender for food microbiologists, used for easy and effective processing of multiple samples, without the risk of cross contamination and the digital control panel provides Simple control of processing features such as paddle speed and processing time, essential for consistent results.

RE301 Digital Rotary Evaporator-Yamato

This rotavap with digital control pane has the essential features, which give you the freedom and convenience you need at the lab. With one touch, you can easily lower or raise this digital rotary evaporator because of its motorized lift. You can also quickly adjust its rotary joint within 80mm and remove the flask by turning the flask release nut.

Features:

• Available in BRC with an oil bath
• Stable rotation at low and high speeds
• Unique durable vacuum seal for up to 2L flasks
• One-touch electric lift for easy up-and-down movement
• Digital display of rotary evaporator’s motor speed (rpm)
• Removable bath for easy cleaning and water replacement
• Easy-to-remove evaporation flask via built-in flask remover
• Anti-liquid stagnation and backflow glass condenser design
• Faster distillation
• Adjustable rotary joint locking position within 80 mm.

Labconco™ FreeZone™ 2.5L −84°C Benchtop Freeze Dryers

FreeZone Benchtop Freeze Dryers are designed for lyophilizing light to moderate sample loads; Freeze-drying is a process typically used to remove residual solvent from a material to produce a dry powder.

Specifications:

• Type: Freeze Dryer/Lyophilizer
• Capacity (Metric): 2.5 L (before defrosting)
• Temperature Range (Metric): -84°C
• Temperature Range (English): -119°C